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px459 2 0 mammalian expression vector  (Addgene inc)


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    Structured Review

    Addgene inc px459 2 0 mammalian expression vector
    Px459 2 0 Mammalian Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 2902 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px459+2+0+mammalian+expression+vector/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/bio_rxiv__2025__02__12__637879-96-15-20
    Average 96 stars, based on 2902 article reviews
    px459 2 0 mammalian expression vector - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    CRISPR:

    Article Title: Activity of the SPCA1 calcium pump couples sphingomyelin synthesis to sorting of secretory proteins in the trans -Golgi network
    Article Snippet: .. For CRISPR/Cas9 targeting guide RNA oligos and their complementary sequences were cloned with suitable overhangs into a pX459 2.0 mammalian expression vector (Addgene plasmid # 62988) as described in ( Ran et al., 2013 ). .. DNA oligonucleotides encoding CRISPR/Cas9 gRNA sequences were synthesized by Yale Keck Oligonucleotide Synthesis facility or by metabion international AG (Planegg, Germany).

    Article Title: Cholesterol depletion activates trafficking-coupled sphingolipid synthesis
    Article Snippet: .. Two guide RNA (targeting sequences: 5’-tcggatgtaattggcaaacc and 5’-ctcttccgattacctgctgg) were each inserted into the CRISPR/Cas9-expressing plasmid, pX459 2.0 mammalian expression vector (Addgene plasmid # 62988). .. Methyl-β-cyclodextrin (MβCD, 377110050) and (2-hydroxypropyl)-β -cyclodextrin (HPCD, H31133.06) were purchased from Millipore Sigma.

    Clone Assay:

    Article Title: Activity of the SPCA1 calcium pump couples sphingomyelin synthesis to sorting of secretory proteins in the trans -Golgi network
    Article Snippet: .. For CRISPR/Cas9 targeting guide RNA oligos and their complementary sequences were cloned with suitable overhangs into a pX459 2.0 mammalian expression vector (Addgene plasmid # 62988) as described in ( Ran et al., 2013 ). .. DNA oligonucleotides encoding CRISPR/Cas9 gRNA sequences were synthesized by Yale Keck Oligonucleotide Synthesis facility or by metabion international AG (Planegg, Germany).

    Expressing:

    Article Title: Activity of the SPCA1 calcium pump couples sphingomyelin synthesis to sorting of secretory proteins in the trans -Golgi network
    Article Snippet: .. For CRISPR/Cas9 targeting guide RNA oligos and their complementary sequences were cloned with suitable overhangs into a pX459 2.0 mammalian expression vector (Addgene plasmid # 62988) as described in ( Ran et al., 2013 ). .. DNA oligonucleotides encoding CRISPR/Cas9 gRNA sequences were synthesized by Yale Keck Oligonucleotide Synthesis facility or by metabion international AG (Planegg, Germany).

    Article Title: Cholesterol depletion activates trafficking-coupled sphingolipid synthesis
    Article Snippet: .. Two guide RNA (targeting sequences: 5’-tcggatgtaattggcaaacc and 5’-ctcttccgattacctgctgg) were each inserted into the CRISPR/Cas9-expressing plasmid, pX459 2.0 mammalian expression vector (Addgene plasmid # 62988). .. Methyl-β-cyclodextrin (MβCD, 377110050) and (2-hydroxypropyl)-β -cyclodextrin (HPCD, H31133.06) were purchased from Millipore Sigma.

    Plasmid Preparation:

    Article Title: Activity of the SPCA1 calcium pump couples sphingomyelin synthesis to sorting of secretory proteins in the trans -Golgi network
    Article Snippet: .. For CRISPR/Cas9 targeting guide RNA oligos and their complementary sequences were cloned with suitable overhangs into a pX459 2.0 mammalian expression vector (Addgene plasmid # 62988) as described in ( Ran et al., 2013 ). .. DNA oligonucleotides encoding CRISPR/Cas9 gRNA sequences were synthesized by Yale Keck Oligonucleotide Synthesis facility or by metabion international AG (Planegg, Germany).

    Article Title: Cholesterol depletion activates trafficking-coupled sphingolipid synthesis
    Article Snippet: .. Two guide RNA (targeting sequences: 5’-tcggatgtaattggcaaacc and 5’-ctcttccgattacctgctgg) were each inserted into the CRISPR/Cas9-expressing plasmid, pX459 2.0 mammalian expression vector (Addgene plasmid # 62988). .. Methyl-β-cyclodextrin (MβCD, 377110050) and (2-hydroxypropyl)-β -cyclodextrin (HPCD, H31133.06) were purchased from Millipore Sigma.



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    96
    Addgene inc px459 2 0 mammalian expression vector
    Px459 2 0 Mammalian Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px459+2+0+mammalian+expression+vector/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/bio_rxiv__2025__02__12__637879-96-15-20
    Average 96 stars, based on 1 article reviews
    px459 2 0 mammalian expression vector - by Bioz Stars, 2026-09
    96/100 stars
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